Molecular genetic diversity of prunus armeniaca L. (Rosaceae) genotypes by RAPD, ISSR-PCR, and chloroplast DNA (cpDNA) trnL-F sequences
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2020Metadata
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Sevindik, E., Murathan, Z., T., Sevindik, M., (2020). Molecular genetic diversity of prunus armeniaca L. (Rosaceae) genotypes by RAPD, ISSR-PCR, and chloroplast DNA (cpDNA) trnL-F sequences. International Journal of Fruit Science, 20(3), 1652-S1661. DOI: 10.1080/15538362.2020.1828223Abstract
In this study, phylogenetic analysis of some TurkishPrunus armeniacaL. genotypes was conducted based on RAPD-PCR, ISSR-PCR, and chloroplast DNA (trnL-F) sequence analyses.Prunus armeniacagenotypes were obtained from Malatya province, and brought to the laboratory for genomic DNA isolation. Eleven RAPD and fifteen ISSR primers were used to determine molecular characterization ofPrunus armeniacagenotypes. For the amplification of thetrnL-F region,trne andtrnf primers were used in Polymerase Chain Reaction (PCR). A total of 46 and 95 bands were obtained with RAPD and ISSR analysis, respectively. ThetrnL-F sequences varied from 398 to 403 nucleotides. Average nucleotide composition oftrnL-F sequences was 31.0% T, 14.4% C, 37.4% A and 17.2% G. The genetic distance between the five apricot genotypes is 0.00. A maximum likelihood tree was generated to determine genetic relationships amongPrunus armeniacagenotypes. Analysis ofPrunus armeniacagenotypes based on RAPD, ISSR, andtrnL-F sequences revealed that the rate of polymorphism obtained using RAPD and ISSR were higher than those obtained through thetrnL-F sequences.