TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF CAMPANULA STRIGOSA BANKS & SOL

Abstract

Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant and antibacterial properties. Total phenolic and flavonoid concentrations were also calculated. For this purpose, a soxhlet apparatus was used to extract ethanol and methanol from the plant's above-ground portions. The effectiveness of antioxidants was measured using Rel Assay kits. The agar dilution technique was used to test for antimicrobial activity against various bacterial and fungus species. Folin-Ciocalteu reagent was used to quantify total phenolic content. An aluminium chloride assay determined the total flavonoid concentration. Studies found that the plant extract had a TAS value of 4.974 +/- 0.259 mmol/L, a TOS value of 12.437 +/- 0.150 mu mol/L, and an OSI value of 0.251 +/- 0.014. The plant extract was shown to be effective against bacterial strains at concentrations between 100 and 400 mu g/mL, and against fungal strains at concentrations between 50 and 100 mu g/mL. Ethanol extract also had a greater total phenolic concentration and total flavonoid content. C. strigosa was shown to have antibacterial and antioxidant properties in this study.