The Effect of Saturated Atmosphere Spray and Touching Methods Used Conditioning of Honey Bee’s on Proboscis Eject Reflections (PER)

Abstract

It has been done conditioning experience by observing proboscis eject reflections (PER) and stingejections (SER) on honey bees. Today, many scientists have showed that honey bees can be usedby conditioning for the determination of events such as narcotics, mines, diseases, magnetic fieldsin the experiment (test). Saturated atmosphere spray and touching methods have been mostly usedfor studying of proboscis eject reflections (PER). This study was done with different honey beeraces and ecotypes and in this study, aromatic plant extract containing 50 cc of atmospheric scentwere used for saturated atmosphere sprey's conditioning method. The worker bees in theconditioning chamber have been presented by injecting to head level from the 5 centimeterdistance approximately in 3 seconds and then they have been rewarded with a syrup mixture ofcontaining a split a (1/1 w/w) sugar and water. In the touching conditioning method, by thetouching of worker – bee, antennas with a wooden toothpick dips to oil aromatic plant extract. Asingle toothpick has been used for every worker bee that has been recorded whether it has showedor not proboscis eject reflections (PER) by repeating tree times of these processes with a quarterbreaks. In the experiment, it has been observed that all the races and ecotypes of honey bees usedin the experiment have showed conditioning in both two methods in the statistical analysis of therecorded data. An important difference has been observed in the conditioning and rememberingamong the application methods. At the end of the experiment, it has been observed that touchingapplication has more conditioning and remembering rates than saturated spray application inconditioning and remembering. In statistical meaning, these two applications have occurred in twodifferent groups.