Antioxidant, oxidant potentials and element content of edible wild mushroom Helvella leucopus

Abstract

This study aims to determine the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), DPPH free radical scavenging activity and element levels of the mushroom Helvella leucopus Pers. Ethanol, methanol and dichloromethane extracts of the mushroom samples were obtained using a Soxhlet device. TAS, TOS and OSI values were determined using Rel Assay kits. Cr, Cu, Mn, Fe, Ni, Cd, Pb, and Zn contents were determined using an atomic absorption spectrophotometer. Consequently, this study has demonstrated that H. leucopus has antioxidant potential. As a result of the studies, TAS value of the mushroom was found as 2.181 +/- 0.069 mmol/L, TOS value was 14.389 +/- 0.170 mu mol/L and OSI value was 0.661 +/- 0.022. DPPH free radical activity was determined as EtOH extract 49.80 +/- 0.71, MeOH extract 44.98 +/- 2.18 and DCM extract 23.68 +/- 1.37 in 1 mg/mL extract concentration of mushroom. In addition, it was found that the mushroom contains high levels of Pb and Mn. In conclusion, it was found that H. leucopus could be used as a natural agent in pharmacological designs due to its antioxidant activity.