Genetic Relationship of Seven Endemic Inula L. (Asteraceae) Species Grown in Turkey

Abstract

In this study, genetic relationship of ISSR markers of seven endemic Inula species distributed in\rTurkey was carried out. Plant samples were collected from different regions of Turkey in 2013 and\rgDNA was obtained by DNA isolation from green leaves. Genetic relationship between species was\rdetermined using 12 ISSR primers. PCR products were run on agarose gel electrophoresis and\rvisualized under UV light. All gel images were examined and the presence and absence of\rpolymorphic bands were scored as 0 and 1. A total of 85 bands were obtained from the primers. Of\rthese, 74 polymorphic and 11 monomorphic bands were obtained. The total polymorphism rate was\rfound to be approximately 87.05%. The phylogenetic tree and genetic distances between species\rwere calculated using the PAUP 0 4.0b10 analysis program. According to the distance matrix, the\rgenetic distance was found between the closest Inula helenium subsp. orgyalis and Inula helenium\rsubsp. vanensis (0.29851), while the farthest between Inula sarana and Inula macrocephala\r(0.56000) species. The phylogenetic tree was obtained using the UPGMA algorithm, and the tree\rconsisted of two groups. The results were compared with the morphological, palynological nrDNA\rand cpDNA results of the past. Our findings supported previous studies