Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Abstract

Lamiaceae family includes annual and perennial 250 genus which are commonly used for their medicinal aromatic properties due to its high biologically active compounds. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum (Telbalıcak) is a member of Lamium genus spread over in Turkey. Lamium species are used for cure since ancient times. Tissue culture provides important advantages for the conservation and sustainability of genetic resources such as clonal and mass production in a limited area. Micropropogation is one of the most significant alternatives for rapid clonal propagation in a short time. In this study, nodes include one or two leaves were placed on MS medium including different levels of BA and GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) for propagation. The explants regenerated to the shoots with the response of 82.5% and 80% in MS medium including 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 respectively. Rooting experiment was carried on MS medium supplied with 0, 0.5, 1, 1.5, 2 mg/L Indole-3- Butyric Acid (IBA) for root induction. Maximum root formation was observed as 45% on MS medium including 0.5 mg/L IBA. Plantlets were acclimatized and 80% of the plants are survived after acclimatization.