Investigation of the polymorphism of the myostatin gene by using PCR-RFLP in two tilapia species (Oreochromis aureus and oreochromis niloticus)

Abstract

The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis. © 2015, World Food Ltd. and WFL Publishers. All rights reserved.