Effects of single and binary applications of bisphenol A and NaCl on Ceratopyllum demersum

Abstract

Determining the physiological effects of single and binary applications of BPA and NaCl on the Ceratophyllum demersum L., which is a rootless submerged aquatic macrophyte, is the aim of the present study. For this purpose, the macrophyte was collected from a local pond that was not exposed to any pollution. Macrophytes were acclimatized in 10% nutrient solution for 14 days, than they were treated with 17.2 mg/L BPA; 10 and 100 mM NaCl; 17.2 mg/L BPA plus 10 mM NaCl and 17.2 mg/L BPA plus 100 mM NaCl. The macrophyte without added BPA or/and NaCl served as control (without treatment). Increasing concentrations of NaCl induced a progressive accumulation of Na in the macrophyte tissues. On the other hand, BPA application partially reduced Na uptake. Nutrient uptake was affected differently by the applications. The contents of some nutrients such as Cu, Zn, Mn, K and Mg were generally reduced, whereas the Fe and Ca contents were increased. In general, increases in contents of total carbohydrate, total phenolic and non-protein sulfhydryl groups were found, when compared to control. Protein and photosynthetic pigment contents, on the contrary to these, were decreased. According to findings, the increase in H2O2 and MDA levels showed that single and combined applications of BPA and NaCl in C. demersum tissues induced oxidative stress.